Milk N-acetyl-B-D-glucosaminidase and serum albumin as indicators of subclinical mastitis in the camel

NAGase activity (NAGase) and serum albumin concentrations were determined in milk from 101 traditionally managed camels in the Sudan. NAGase, a lysosomal enzyme released from damaged epithelial cells as well as other somatic cells in milk, was recorded for quarter milk samples from camels (n = 353) using the fluoroscan method. NAGase activity in major pathogen-infected quarters was significantly (P < 0.05) higher than in minor pathogen-infected and non-infected quarters. Means for minor pathogen-infected quarters were also significantly higher than non-infected quarters. Concentrations of serum albumin, resulting from increased vascular permeability, in quarter milk samples (n = 320) were measured by the radial immuno-diffusion test. Serum albumin content in milk was not affected by infection status of the quarter. There were, however, large variations in serum albumin levels and, to a lesser extent, NAGase values between samples in both infected and non-infected quarters. NAGase was more effective in predicting bacteriological status of the quarter than serum albumin.