SYNTHESIS OF PHOSPHATIDYLCHOLINE WITH (N-3) FATTY-ACIDS BY PHOSPHOLIPASE A-2 IN MICROEMULSION

被引:52
作者
NA, A
ERIKSSON, C
ERIKSSON, SG
OSTERBERG, E
HOLMBERG, K
机构
[1] SWEDISH INST FOOD RES,S-44485 STENUGSUND,SWEDEN
[2] BEROL NOBEL AB,S-44485 STENUNGSUND,SWEDEN
关键词
AOT; DHA; EPA; microemulsion; phosphatidylcholine; phospholipase A 2; synthesis;
D O I
10.1007/BF02540488
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Phosphatidylcholine containing a long chain polyunsaturated acyl group at the 2-position has been prepared by phospholipase A 2 catalyzed esterification of lysophosphatidylcholine with polyunsaturated fatty acids EPA (C20:5) or DHA (C22:6). Preliminary studies showed that the other fatty acids, such as lauric acid (C12), palmitic acid (C16), stearic acid (C18) and linoleic acid (C18:2), were also incorporated. To our knowledge, phospholipase A 2 catalyzed condensation reactions have not been reported in the literature before. The reactions were performed in sodium bis(2-ethylhexyl)-sulfosuccinate-based microemulsions containing small amounts of water. Synthesis of the same phospholipid by transesterification of phosphatidylcholine with the polyunsaturated acids in microemulsion failed; however, enzymatic hydrolysis to lysophosphatidylcholine was facile, quantitative conversion from phosphatidylcholine being attained after 16 hr reaction time. An additional observation was that, unlike enzymatic hydrolysis of phospholipids, the condensation reaction catalyzed by phospholipase A 2 was totally independent of the presence of calcium. © 1990 AOCS Press.
引用
收藏
页码:766 / 770
页数:5
相关论文
共 21 条
  • [1] BOX GEP, 1978, STATISTICS EXPT
  • [2] FISH OIL INCREASES PERIPHERAL CAPILLARY BLOOD-CELL VELOCITY IN HUMANS
    BRUCKNER, G
    WEBB, P
    GREENWELL, L
    CHOW, C
    RICHARDSON, D
    [J]. ATHEROSCLEROSIS, 1987, 66 (03) : 237 - 245
  • [3] EFFECTS OF DIETS ENRICHED IN EICOSAPENTAENOIC OR DOCOSAHEXAENOIC ACIDS ON PROSTANOID METABOLISM IN THE RAT
    CROFT, KD
    BEILIN, LJ
    LEGGE, FM
    VANDONGEN, R
    [J]. LIPIDS, 1987, 22 (09) : 647 - 650
  • [4] DYERBERG J, 1986, NUTR REV, V44, P125, DOI 10.1111/j.1753-4887.1986.tb07603.x
  • [5] EKSTRAND B, 1988, Patent No. 88020953
  • [6] Holmberg K, 1987, PROG COLLOID POLYM S, V74, P98
  • [7] ENZYMES HOSTED IN REVERSE MICELLES IN HYDROCARBON SOLUTION
    LUISI, PL
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1985, 24 (06): : 439 - 450
  • [8] A METHOD FOR THE SYNTHESIS OF ISOMERICALLY PURE SATURATED MIXED-CHAIN PHOSPHATIDYLCHOLINES
    MASON, JT
    BROCCOLI, AV
    HUANG, CH
    [J]. ANALYTICAL BIOCHEMISTRY, 1981, 113 (01) : 96 - 101
  • [9] SYNTHESIS OF TRIACYLGLYCEROL BY LIPASE IN PHOSPHATIDYLCHOLINE REVERSE MICELLAR SYSTEM
    MORITA, S
    NARITA, H
    MATOBA, T
    KITO, M
    [J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1984, 61 (10) : 1571 - 1574
  • [10] MORRISON WR, 1964, J LIPID RES, V5, P600