CONDITIONS FOR QUANTITATIVE TRANSFORMATION IN ACINETOBACTER-CALCOACETICUS

被引：58

作者：

CRUZE, JA ^{[1
]}

SINGER, JT ^{[1
]}

FINNERTY, WR ^{[1
]}

机构：

[1] UNIV GEORGIA,DEPT MICROBIOL,ATHENS,GA 30602

来源：

CURRENT MICROBIOLOGY | 1979年 / 3卷 / 03期

关键词：

D O I：

10.1007/BF02601853

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A transformation procedure that yielded high efficiencies was developed for Acinetobacter calcoaceticus. Strain BD413 Ura−trpE was transformed to tryptophan prototrophy using highly purified DNA. Experimental parameters studied were: (i) recipient cell concentration, (ii) DNA concentration, (iii) growth phase of the recipient cell population, (iv) composition of the growth and transforming medium, and (v) time of incubation of recipient cells with donor DNA. Strain BD413 was competent for transformation throught the growth cycle, with highest competence occurring early in the exponential phase of growth. Maximal transformation efficiencies of 0.5% to 0.7% were obtained in media supporting rapid growth. Recipient cell concentrations of 1×106 to 6×106 cells/ml yielded the highest transformation frequencies, regardless of DNA concentration. © 1979, Springer-Verlag New York Inc. All rights reserved.

引用

页码：129 / 132

页数：4

共 6 条

[1]

AHLQUIST EF, 1975, J GEN MICROBIOL, V91, P338, DOI 10.1099/00221287-91-2-338

[2] STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID [J].