THE POLYMERASE CHAIN-REACTION AND POSTMORTEM FORENSIC IDENTITY TESTING - APPLICATION OF AMPLIFIED D1S80 AND HLA-DQ-ALPHA LOCI TO THE IDENTIFICATION OF FIRE VICTIMS

被引：62

作者：

SAJANTILA, A

STROM, M

BUDOWLE, B

KARHUNEN, PJ

PELTONEN, L

机构：

[1] UNIV HELSINKI,DEPT FORENS MED,SF-00300 HELSINKI,FINLAND

[2] FED BUR INVEST ACAD,CTR FORENS SCI RES & TRAINING,QUANTICO,VA 22135

来源：

FORENSIC SCIENCE INTERNATIONAL | 1991年 / 51卷 / 01期

关键词：

FORENSIC SCIENCE; POLYMERASE CHAIN REACTION; FIRE VICTIMS; IDENTITY TESTING; POSTMORTEM;

D O I：

10.1016/0379-0738(91)90203-U

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

The application of DNA typing methods after amplification by the polymerase chain reaction (PCR) of DNA derived from body tissues from charred fire victims was investigated. A total of 26 different tissue specimens from ten extensively burned individuals were analyzed. The samples included femoral muscle, psoas muscle, bone marrow and blood. The post-mortem period varied from 38 to 183 h. After amplifying the DNA by PCR from the various tissues, the D1S80 locus was analyzed with a high resolution polyacrylamide gel electrophoresis technique followed by silver staining and the alleles of the HLA-DQ-alpha locus were detected by using a reverse dot blot format. All samples could be typed for both loci and the genotypes were consistent in the various tissues from each individual. A parentage test was performed in two cases and Mendelian inheritance of the alleles for both loci was observed.

引用

页码：23 / 34

页数：12

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