DETECTION OF SALMONELLA SPP IN OYSTERS BY PCR

被引：97

作者：

BEJ, AK

MAHBUBANI, MH

BOYCE, MJ

ATLAS, RM

机构：

[1] UNIV LOUISVILLE,DEPT BIOL,139 LIFE SCI BLDG,BELKNAP CAMPUS,LOUISVILLE,KY 40292

[2] UNIV ALABAMA,DEPT BIOL,BIRMINGHAM,AL 35294

[3] UNIV ALABAMA,SCH DENT,BIRMINGHAM,AL 35294

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 01期

关键词：

D O I：

10.1128/AEM.60.1.368-373.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

PCR DNA amplification of a region of the himA gene of Salmonella typhimurium specifically detected Salmonella spp. In oysters, 1 to 10 cells of Salmonella spp. were rapidly detected by the PCR following a preenrichment step to increase sensitivity and to ensure that detection was based on the presence of viable Salmonella spp.

引用

页码：368 / 373

页数：6

共 31 条

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[2]

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[3]

ATMER RL, 1993, APPL ENVIRON MICROB, V59, P631

[4]

Ausubel FM., 1995, MOL REPROD DEV, V3rd edn, DOI DOI 10.1002/MRD.1080010210