EFFECTS OF DOXORUBICIN ON EXCITATION-CONTRACTION COUPLING IN GUINEA-PIG VENTRICULAR MYOCARDIUM

Doxorubicin, an anticancer drug, was recently shown to release Ca2+ from cardiac sarcoplasmic reticulum (SR) by increasing the open probability of Ca2+ release channels. In the present study, we investigated the effects of doxorubicin on excitation-contraction coupling of guinea pig heart preparations. In papillary muscles contracting at 0.5 Hz, 100 mu mol/L doxorubicin produced within 3 hours the following effects: it increased the force of contraction by 269.3+/-19.8% (n=6) and prolonged the time to peak force by 75.1+/-8.7% (n=6), relaxation time by 54.7+/-8.7% (n=6), and action potential duration (APD) at 90% repolarization (APD(90)) by 38.6+/-2.9% (n=3). Despite its positive inotropic effect, doxorubicin depressed the early contraction component by increasing the latency between stimulus and the onset of force development. In single myocytes, 100 mu mol/L doxorubicin prolonged APD(90) by 62.1% (n=18) and blocked time-dependent delayed rectifier K+ current (I-K) by 44% (n=9). Ca2+ inward current and inward rectifier K+ current were not affected by doxorubicin. Ca2+ transients elicited in myocytes loaded with the fluorescent Ca2+ indicator fura 2 were strongly suppressed by doxorubicin in their initial rising phase. There after, doxorubicin produced a delayed rise in intracellular Ca2+, which reached a late peak exceeding that of the control peak by 52+/-8% (n=5). The results suggest that doxorubicin decreases Ca2+-induced Ca2+ release from cardiac SR, probably by increasing the SR Ca2+ leak. On the other hand, prolongation of APD due to inhibition of I-K allows more Ca2+ to enter the cell. After being only temporarily buffered by the SR, Ca2+ may accumulate in the cytosol as long as depolarization is maintained and lead to a more complete activation of contractile proteins.