CHEMISTRY AND BIOLOGY OF PHOSPHOLIPIDS FROM AN UNCLASSIFIED MYCOBACTERIA P6

被引:12
作者
MOTOMIYA, M
MAYAMA, A
FUJIMOTO, M
SATO, H
OKA, S
机构
[1] The Research Institute for Tuberculosis, Leprosy and Cancer Tohoku University, Sendai
关键词
D O I
10.1016/0009-3084(69)90007-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Crude phospholipid fraction from P6 (Scotochromogen) prepared by extraction with chloroform: methanol (2:1), followed by removal of non-lipid contaminants by Folch's procedure and extraction with acetone, yielded three fractions by column chromatography with silicic acid. These fractions were designated as fractions I, II and III in the decreasing order of their Rf's on thin layer plates of Silica Gel. Fraction I was identified as diphosphatidyl glycerol (cardiolipin) and was antigenic in flocculation test for syphilis and in latex agglutination test for lepromatous leprosy. Fraction II was identified as phosphatidyl ethanolamine and Fraction III as phosphatidyl inositol monomannoside. © 1969.
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页码:159 / &
相关论文
共 35 条
[1]  
ABRAMSON D, 1964, J LIPID RES, V5, P628
[2]   SEPARATION AND ANALYSES OF INDIVIDUAL PHOSPHOLIPIDS OF MYCOBACTERIA [J].
AKAMATSU, Y ;
NOJIMA, S .
JOURNAL OF BIOCHEMISTRY, 1965, 57 (03) :430-+
[3]   The estimation of phosphorus [J].
Allen, RJL .
BIOCHEMICAL JOURNAL, 1940, 34 (06) :858-865
[4]  
ANSELL GB, 1964, BBA LOBRARY, V3, P99
[5]  
BLOCK RJ, 1958, MANUAL PAPER CHROMAT, P149
[6]  
BOHM P, 1954, Z PHYSIOL CHEM, V298, P110
[7]  
BRENNAN P, 1967, J BIOL CHEM, V242, P3046
[9]  
DITTMER JC, 1964, J LIPID RES, V5, P126
[10]  
DUBOCZY BO, 1966, AM REV RESPIR DIS, V94, P914