INTERACTION OF DNA WITH DNA-BINDING PROTEINS - PROTEIN EXCHANGE AND COMPLEX STABILITY

被引：20

作者：

GEIDER, K

机构：

[1] Abteilung Moekulare Biologie, Maz-Planck-Institut für Medizinische Forrschung, Heidelberg, D-6900

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1978年 / 87卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1978.tb12414.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Competition of the DNA‐binding proteins I and II of Escherichia coli and of the phage fd DNAbinding protein for single‐stranded DNA was investigated. Their roles in cells might be judged from thier binding affinited to DNA and their mutual exchange in the DNA ‐binding protein II displaced half of the protein I in the complex with singel‐stranded DNA when no double stranded DNA was present. SProtein‐complexd single strands were protected against degraddaiton. The protection is less pronuounced for protein II which can increase the stability of the fd DNA complex with DNA‐binding protein I against nucleolytic celevage. Copyright © 1978, Wiley Blackwell. All rights reserved

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页码：617 / 622

页数：6

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