HIGH-MOLECULAR-WEIGHT NUCLEAR POLYADENYLATE-CONTAINING RIBONUCLEIC-ACID ISOLATED FROM THE LACTATING GUINEA-PIG MAMMARY-GLAND CONTAINS MILK-PROTEIN MESSENGER RIBONUCLEIC-ACID SEQUENCES

被引：3

作者：

BATHURST, IC ^{[1
]}

CRAIG, RK ^{[1
]}

CAMPBELL, PN ^{[1
]}

机构：

[1] MIDDLESEX HOSP,SCH MED,COURTAULD INST BIOCHEM,LONDON W1P 7PN,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1979年 / 181卷 / 02期

关键词：

D O I：

10.1042/bj1810501

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Steady-state poly(A)-containing nuclear RNA was isolated from the lactating guinea-pig mammary gland and analysed by sucrose-gradient centrifugation under denaturing conditions. 2. Nucleic acid-hybridization studies demonstrated the presence of small amounts of high-molecular-weight RNA species containing milk-protein mRNA sequences sedimenting at 25S. These were not found in the post-nuclear supernatant, where milk-protein sequences sedimented only between 12S and 15S; these were also the predominant species in the nuclear fraction. 3. The results are consistent with the transcription of milk-protein genes as high-molecular-weight precursor RNA species, 3-4 times as large as the active mRNA species isolated from the post-nuclear fraction.

引用

页码：501 / 504

页数：4

共 16 条

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