TRANSFORMING GROWTH FACTOR-BETA-1 STIMULATES PROLIFERATION OF MORRIS HEPATOMA-CELLS IN SERUM-FREE SOFT AGAR CULTURE SYSTEM SUPPLEMENTED WITH EGF AND INSULIN

被引：4

作者：

GUZIK, K ^{[1
]}

KLEIN, A ^{[1
]}

机构：

[1] JAGIELLONIAN UNIV,INST MOLEC BIOL,DEPT ANIM BIOCHEM,MICKIEWICZA STR 3,PL-31120 KRAKOW,POLAND

来源：

CANCER LETTERS | 1990年 / 54卷 / 1-2期

关键词：

anchorage-independent growth; growth factors; Morris hepatoma; serum-free culture;

D O I：

10.1016/0304-3835(90)90090-K

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The anchorage-independent growth of Morris hepatoma 7777 (MH) cells in serum-free medium was examined. The influence of insulin, epidermal growth factor and transforming growth factor β1 (TGF-β1) on [3H]thymidine incorporation and colony formation of the investigated cells was described. Contrary to normal rat hepatocytes TGF-β1 plus EGF and insulin were found to stimulate MH cells proliferation in presented conditions. A simple, chemically-defined culture system suitable for research on mitogenic peptides was proposed. © 1990.

引用

页码：51 / 56

页数：6

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