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HIGH-EFFICIENCY ELECTROPORATION OF PSEUDOMONAS-AERUGINOSA USING FROZEN CELL-SUSPENSIONS

被引：93
作者：
FARINHA, MA [1 ]
KROPINSKI, AM [1 ]
机构：
[1] QUEENS UNIV,DEPT MICROBIOL & IMMUNOL,KINGSTON K7L 3N6,ONTARIO,CANADA
来源：
FEMS MICROBIOLOGY LETTERS | 1990年 / 70卷 / 02期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Bacterial genetics; Electroporation Transformation; Plasmid; Pseudomonas; Recombinant DNA;
D O I：
10.1016/S0378-1097(05)80043-6
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
High efficiency transformation of Pseudomonas aeruginosa was achieved using frozen cell suspension and high voltage electroporation. We have obtained frequencies as high as 5.8 × 103 transformants/g of plasmid DNA using PA01 strain OT684 and a buffer of 15% glycerol-1 mM MOPS. The method allows for easy and reproducible production of frozen cell suspensions for rapid transformation of P. aeruginosa. © 1990 Federation of European Microbiological Societies.
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页码：221 / 226
页数：6
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