CONFOCAL IMAGING OF IONIZED CALCIUM IN LIVING PLANT-CELLS

被引：34

作者：

WILLIAMS, DA ^{[1
]}

CODY, SH ^{[1
]}

GEHRING, CA ^{[1
]}

PARISH, RW ^{[1
]}

HARRIS, PJ ^{[1
]}

机构：

[1] LA TROBE UNIV,DEPT BOT,BUNDOORA,VIC 3083,AUSTRALIA

来源：

CELL CALCIUM | 1990年 / 11卷 / 04期

关键词：

D O I：

10.1016/0143-4160(90)90006-G

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Laser-scanning confocal microscopy has been used in conjunction with Fluo-3, a highly fluorescent visible wavelength probe for Ca2+, to visualise Ca2+-dynamics in the function of living plant cells. This combination has overcome many of the problems that have limited the use of fluorescence imaging techniques in the study of the role of cations (Ca2+ and H+) in plant cell physiology and enables these processes to be studied in single cells within intact plant tissue preparations. Maize coleoptiles respond to application of ionophores and plant growth hormones with elevations in cytosolic Ca2+ that can be resolved with a high degree of spatial resolution and can be interpreted quantitatively. © 1990.

引用

页码：291 / 297

页数：7

共 24 条

[1] A GRADIENT OF CYTOPLASMIC FREE CALCIUM IN GROWING RHIZOID CELLS OF FUCUS-SERRATUS [J].