ACTIVATION OF HUMAN NEUTROPHILS BY TYPE-I COLLAGEN - REQUIREMENT OF 2 DIFFERENT SEQUENCES

被引：43

作者：

MONBOISSE, JC ^{[1
]}

BELLON, G ^{[1
]}

RANDOUX, A ^{[1
]}

DUFER, J ^{[1
]}

BOREL, JP ^{[1
]}

机构：

[1] INST JEAN GODINOT,HEMATOL LAB,F-51062 REIMS,FRANCE

来源：

BIOCHEMICAL JOURNAL | 1990年 / 270卷 / 02期

关键词：

D O I：

10.1042/bj2700459

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Contact between type I collagen purified from several species and human polymorphonuclear neutrophils (PMNs) triggers the production of O2.- by these cells. The activity of collagen is located in the α1(I)-CB6 cyanogen bromide-cleaved (CB)-peptide, which is the C-terminal CB-peptide of the α1(I) chain. Experiments based on the competitive inhibition of O2.- production by simultaneous incubation of PMNs with type I collagen and synthetic peptides identical to the conserved sequences of this collagen demonstrated that the binding of collagen to PMNs and the subsequent activation of these cells depend on the simultaneous presence of two sequences: Arg-Gly-Asp [residues 915, 916 and 917 of the complete α1(I) chain, located in the helical part] Asp-Gly-Gly-Arg-Tyr-Tyr (residues 1034-1039, located in the C-terminal non-helical telopeptide).

引用

页码：459 / 462

页数：4

共 13 条

[1] BIOLOGICAL DEFENSE MECHANISMS - PRODUCTION BY LEUKOCYTES OF SUPEROXIDE A POTENTIAL BACTERICIDAL AGENT [J].