GTP GAMMA-S-DEPENDENT REGULATION OF SMOOTH-MUSCLE CONTRACTILE ELEMENTS

被引:124
作者
KUBOTA, Y
NOMURA, M
KAMM, KE
MUMBY, MC
STULL, JT
机构
[1] UNIV TEXAS,SW MED CTR,DEPT PHYSIOL,DALLAS,TX 75235
[2] UNIV TEXAS,SW MED CTR,MOSS HEART CTR,DALLAS,TX 75235
[3] UNIV TEXAS,SW MED CTR,DEPT PHARMACOL,DALLAS,TX 75235
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 262卷 / 02期
关键词
MYOSIN PHOSPHORYLATION; CALCIUM SENSITIVITY; PROTEIN PHOSPHATASE; MYOSIN LIGHT CHAIN KINASE;
D O I
10.1152/ajpcell.1992.262.2.C405
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Guanosine 5'-O-(3-thiotriphosphate) (GTP-gamma-S) increases the sensitivity of the contractile response to activation by Ca2+ in permeabilized tracheal smooth muscle. Increased tension was associated with a proportional increase in myosin light chain phosphorylation. The site of phosphorylation was determined to be serine-19, which corresponds to the site rapidly phosphorylated by myosin light chain kinase. GTP-gamma-S did not affect the contraction induced by the protein phosphatase inhibitor okadaic acid but did enhance contraction produced by Ca2+-independent myosin light chain kinase. In tracheal homogenates Ca2+-dependent myosin light chain kinase activity was not affected by GTP-gamma-S; however, dephosphorylation of P-32-labeled heavy meromyosin by phosphatase was inhibited. Thus GTP-gamma-S may increase the Ca2+ sensitivity of contractile elements in tracheal smooth muscle by inhibition of protein phosphatase activity toward myosin light chain.
引用
收藏
页码:C405 / C410
页数:6
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