PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE PROVOKES CULTURED RAT CHROMAFFIN CELLS TO SECRETE ADRENALINE

被引：176

作者：

WATANABE, T

MASUO, Y

MATSUMOTO, H

SUZUKI, N

OHTAKI, T

MASUDA, Y

KITADA, C

TSUDA, M

FUJINO, M

机构：

[1] Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd., Tsukuba Ibaraki, 300-42

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)80159-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pituitary adenylate cyclase activating polypeptide (PACAP) provoked the rat chromaffin cells to secrete adrenaline. Within 20 min, the amount of adrenaline secreted by PACAP (10-8M) was as much as that caused by acetylcholine (10-4M). PACAP, but not acetylcholine, induced a long-term (over 120 min) increase in secretion of adrenaline. PACAP also activated adenylate cyclase and elevated cytosolic Ca2+ concentration. Furthermore, we found immunoreactive PACAP and PACAP binding sites in the rat adrenal medulla. These results suggest that PACAP has an important role in stimulating secretion of adrenaline in the adrenal medulla. © 1992 Academic Press, Inc.

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页码：403 / 411

页数：9

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