HUMAN ENDOTHELIAL-CELLS AS AN ALTERNATIVE TO DH82 CELLS FOR ISOLATION OF EHRLICHIA-CHAFFEENSIS, EHRLICHIA-CANIS, AND RICKETTSIA-RICKETTSII

被引:23
作者
DAWSON, JE
CANDAL, FJ
GEORGE, VG
ADES, EW
机构
[1] CTR DIS CONTROL,NATL CTR INFECT DIS,SRP,BIOL PROD BRANCH,ATLANTA,GA 30333
[2] CTR DIS CONTROL,NATL CTR INFECT DIS,VIRAL & RICKETTSIAL ZOONOSES BRANCH,ATLANTA,GA 30333
关键词
HUMAN ENDOTHELIUM; EHRLICHIA; RICKETTSIA; HUMAN EHRLICHIOSIS; CANINE EHRLICHIOSIS; ROCKY MOUNTAIN SPOTTED FEVER;
D O I
10.1159/000163808
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Ehrlichia chaffeensis, etiologic agent of human ehrlichiosis, and Rickettsia rickettsii, etiologic agent of Rocky Mountain spotted fever (RMSF), are both tick-borne agents that cause nonspecific symptoms that may be indistinguishable from each other early in the course of infection. E. canis is a canine pathogen closely related to E. chaffeensis and was initially suspected of being the causative agent of human ehrlichiosis. If a febrile illness is reported, after tick exposure, neither ehrlichiosis nor RMSF can be immediately ruled out. When attempts are made to isolate the agent from blood, a very limited amount of blood is often available; we, therefore, sought a tissue-culture cell line that would support the growth of both R. rickettsii and E. chaffeensis. A newly established human microvascular endothelial immortal cell line (CDC/EU.HMEC-1) was evaluated for supporting the growth of both agents. Our results demonstrate that HMEC-1 supports the growth of R. rickettsii, E. chaffeensis, and E: canis and may be a useful tool for the isolation of these agents.
引用
收藏
页码:293 / 296
页数:4
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