DUAL ENZYME LABELS FOR SIMULTANEOUS HETEROGENEOUS ENZYME-LINKED COMPETITIVE-BINDING ASSAYS

被引：3

作者：

BUCKWALTER, JM ^{[1
]}

GUO, X ^{[1
]}

MEYERHOFF, ME ^{[1
]}

机构：

[1] UNIV MICHIGAN, DEPT CHEM, ANN ARBOR, MI 48109 USA

来源：

ANALYTICA CHIMICA ACTA | 1994年 / 298卷 / 01期

基金：

美国国家科学基金会;

关键词：

ENZYMATIC METHODS; SIMULTANEOUS DETECTION; ENZYME-LINKED BINDING ASSAYS; VITAMINS; GLUCOSE-6-PHOSPHATE DEHYDROGENASE; BETA-GALACTOSIDASE;

D O I：

10.1016/0003-2670(94)90038-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of two enzyme labels in a dual analyte simultaneous heterogeneous enzyme-linked competitive binding assay is examined. Reaction conditions for monitoring glucose-6-phosphate dehydrogenase (G6PDH) and beta-galactosidase (beta-gal) activities are found which enable the independent measurement of each enzyme activity in the presence of the other. The two enzymes are used as labels in the development of a model simultaneous heterogeneous competitive binding assay for detecting the biotin and vitamin B-12 content of vitamin tablets. The simultaneous assay is shown to exhibit analytical dose-response characteristics comparable to those of the single analyte assays. The potential use of the G6PDH/beta-gal enzyme pair for devising other dual analyte competitive and non-competitive binding assays is discussed.

引用

页码：11 / 18

页数：8

共 37 条

[1] USE OF ESTERS OF N-HYDROXYSUCCINIMIDE IN PEPTIDE SYNTHESIS [J].