STRUCTURAL-ANALYSIS OF CYCLAMEN SEED XYLOGLUCAN OLIGOSACCHARIDES USING CELLULASE DIGESTION AND SPECTROSCOPIC METHODS

被引:17
作者
BRACCINI, I
DUPENHOAT, CH
MICHON, V
GOLDBERG, R
CLOCHARD, M
JARVIS, MC
HUANG, ZH
GAGE, DA
机构
[1] ECOLE NORMALE SUPER,DEPT CHIM,URA 1679,F-75231 PARIS,FRANCE
[2] INST JACQUES MONOD,ENZYMOL MILIEU STRUCT LAB,F-75251 PARIS,FRANCE
[3] PHYSIOL VEGETALE APPL LAB,F-75251 PARIS,FRANCE
[4] UNIV GLASGOW,GLASGOW G12 8QQ,LANARK,SCOTLAND
[5] MICHIGAN STATE UNIV,DEPT BIOCHEM,E LANSING,MI 48824
关键词
XYLOGLUCAN; CELLULASE; CP-MAS C-13 NMR;
D O I
10.1016/0008-6215(95)00156-N
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Xyloglucan polymers have been isolated from cyclamen seeds and characterized by both liquid and CP-MAS C-13 NMR spectroscopy. Treatment of the polysaccharides with cellulase from Trichoderma viride afforded XG oligomers which have been studied with both mass spectrometry and NMR spectroscopy. The repeating unit in the intact polymers and the most abundant hydrolysis product correspond to the Gal(1) Xyl(3) Glc(4) (XXLG) fragment. However, detection of notable amounts of Xyl(3) Glc(4) (XXXG) and Gal(2) Xyl(3) Glc(4) (XLLG) indicates that the galactose distribution in xyloglucan from cyclamen is irregular. FAB-MS analysis of a new derivative, prepared by forming the glycosamine of m-tetrafluoroethoxy aniline, has led to unambiguous sequence information for the XXLG oligomer.
引用
收藏
页码:167 / 181
页数:15
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