HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF HYDROCORTISONE AND METHYLPREDNISOLONE AND THEIR HEMISUCCINATE SALTS

被引：19

作者：

SMITH, MD

HOFFMAN, DJ

机构：

[1] Abbott Laboratories, North Chicago, IL 60064

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 168卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(00)80705-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatographic (HPLC) procedure for the determination of hydrocortisone hemisuccinate and methylprednisolone hemisuccinate and their corresponding free steroids in experimental, lyophilized products of the hemiesters is described. The method is suitable as a stability-indicating assay. HPLC was performed on a 5-μm ODS column, using a mobile phase of 2% glacial acetic acid, 35-40% acetonitrile and 65-60% water and UV detection (242 nm). A comparison of this HPLC method with the U.S.P. blue tetrazolium assay was made. For freshly prepared samples, the sum of the hemisuccinate ester and the 21-hydroxycorticosteroid as determined by HPLC approximates the blue tetrazolium assay values. © 1979.

引用

页码：163 / 169

页数：7

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