ENZYME IMMUNOASSAY OF TESTOSTERONE IN PLASMA, WITH USE OF POLYETHYLENE-GLYCOL TO SEPARATE ANTIBODY-BOUND AND FREE HORMONE

被引：8

作者：

OSTERMAN, TM

JUNTUNEN, KO

GOTHONI, GD

机构：

[1] Department of Immunology, Medica Pharmaceutical Company Ltd., Helsinki

来源：

STEROIDS | 1979年 / 34卷 / 05期

关键词：

D O I：

10.1016/S0039-128X(79)80018-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme immunoassay for [human plasma] testosterone is described which uses a testosterone-3-(carboxymethyl)oxime-horseradish peroxidase conjugate as the label and an antiserum, raised in rabbits, to testosterone-3-(carboxymethyl)oxime-bovine serum albumin cojugate. Polyethylene glycol (Carbowax 6000) is used to separate antibody-bound and free steroid. The assay has a sensitivity of 12 pg/assay tube and satisfies the usual criteria of specificity, precision and accuracy. The results agree well with those obtained with a comparable radioimmunoassay.

引用

页码：575 / 579

页数：5

共 11 条

[1]

BELANGER L, 1976, CLIN CHEM, V22, P198

[2] IMMUNO-ENZYMATIC ASSAY OF CORTISOL USING ESCHERICHIA-COLI BETA-GALACTOSIDASE AS LABEL [J].