BIOSYNTHESIS OF POLYURONIDES IN MUCOR-ROUXII - PARTIAL CHARACTERIZATION OF FUCOSYL TRANSFERASE

被引：6

作者：

CAMACHOAGUERO, S

BALCAZAROROZCO, R

FLORESCARREON, A

机构：

[1] UNIV GUANAJUATO,FAC QUIM,INST INVEST BIOL EXPTL,APARTADO POSTAL 187,GUANAJUATO 36000,MEXICO

[2] INST POLITECN NACL,CINVESTAV,DEPT GENET & BIOL MOLEC,MEXICO CITY 07000,DF,MEXICO

来源：

EXPERIMENTAL MYCOLOGY | 1990年 / 14卷 / 03期

关键词：

acceptors; detergents; fucosyl transferase; membrane-bound enzyme; Mucor rouxii; mucoran; mucoric acid; polyuronides;

D O I：

10.1016/0147-5975(90)90020-T

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Most of the fucosyl transferase activity fromMucor rouxii was detected in a crude membrane fraction. The enzyme transferredl-fucose from GDP-fucose to endogenous and exogenous acceptors. When crude membrane fractions were treated with neutral detergents such as Trition X-100 or Brij 36 T enzyme activity became dependent on exogenous acceptors such as mucoric acid or mucoran. Brij-treated membrane fractions showed maximum fucosyl transferase activity at pH 6.5, and at a temperature between 22 and 28°C. The cations Mn2+, Mg2+, Co2+, Zn2+, Fe2+, and Ca2+ activated the enzyme about twofold. The former was slightly more stimulatory at 4 mM. Km for GDP-fucose was 10 μM. Evidence was obtained that mucoric acid serves as acceptor for fucosyl moieties. Acid hydrolysis of the product synthesized from GDP-fuc by Brij-treated membrane fractions revealed fucose as the major radioactive sugar. © 1990 Academic Press, Inc.

引用

页码：227 / 233

页数：7

共 21 条

[1]

Avalos Lozano M.A, 1989, REV MEX MIC, V5, P43

[2] CHEMISTRY OF SPORE WALL DIFFERENTIATION IN MUCOR ROUXII [J].