INFLUENCE OF CULTURE CONDITIONS ON GROWTH AND PROTECTIVE ANTIGENICITY OF CLOSTRIDIUM-CHAUVOEI

被引：19

作者：

CORTINAS, TI

MICALIZZI, B

DEGUZMAN, AMS

机构：

[1] Facultad de Química, Bioquímicay Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, San Luis

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1994年 / 77卷 / 04期

关键词：

D O I：

10.1111/j.1365-2672.1994.tb03438.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of culture conditions on growth and immunogenicity of Clostridium chauvoei were examined. The pH control and partial feeding of the carbon source at high concentrations were beneficial for growth. The biomass yield was significatively improved, however the butanol concentration reached toxic levels hampering further growth. For each experimental condition the immunogenicity of cells was tested. No differences were found with cells obtained at different temperatures, but it decreased significatively with the partial supply of the carbon source and pH control.

引用

页码：382 / 387

页数：6

共 17 条

[1]

Cato E. P., 1986, BERGEYS MANUAL SYSTE, P1141

[2] PROTECTIVE ANTIGEN OF A HIGHLY IMMUNOGENIC STRAIN OF CLOSTRIDIUM-CHAUVOEI INCLUDING AN EVALUATION OF ITS FLAGELLA AS A PROTECTIVE ANTIGEN [J].

CHANDLER, HM ;

GULASEKHARAM, J .

JOURNAL OF GENERAL MICROBIOLOGY, 1974, 84 (SEP) :128-134

[3] THE DEVELOPMENT OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR MEASURING THE POTENCY OF VACCINES CONTAINING CLOSTRIDIUM-CHAUVOEI ANTIGENS [J].

CRICHTON, R ;

SOLOMON, J ;

BARTON, AM .

BIOLOGICALS, 1990, 18 (01) :49-54

[4] EFFECTS OF IRON LIMITATION ON PRODUCTION OF A SIDEROPHORE, OUTER-MEMBRANE PROTEINS, AND HEMOLYSIN AND ON HYDROPHOBICITY, CELL ADHERENCE, AND LETHALITY FOR MICE OF VIBRIO-PARAHAEMOLYTICUS [J].

DAI, JH ;

LEE, YS ;

WONG, HC .

INFECTION AND IMMUNITY, 1992, 60 (07) :2952-2956

[5]

de Guzman A M, 1988, Rev Latinoam Microbiol, V30, P73

[6] PHOSPHATE STARVATION ENHANCES EXPRESSION OF THE IMMUNODOMINANT 38-KILODALTON PROTEIN ANTIGEN OF MYCOBACTERIUM-TUBERCULOSIS - DEMONSTRATION BY IMMUNOGOLD ELECTRON-MICROSCOPY [J].

ESPITIA, C ;

ELINOS, M ;

HERNANDEZPANDO, R ;

MANCILLA, R .

INFECTION AND IMMUNITY, 1992, 60 (07) :2998-3001

[7] METABOLISM OF RHAMNOSE AND OTHER SUGARS BY STRAINS OF CLOSTRIDIUM-ACETOBUTYLICUM AND OTHER CLOSTRIDIUM SPECIES [J].

FORSBERG, CW ;

DONALDSON, L ;

GIBBINS, LN .

CANADIAN JOURNAL OF MICROBIOLOGY, 1987, 33 (01) :21-26

[8]

HOLMGREN J, 1969, ACTA PATHOL MIC SC, V76, P304

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

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