MONOCLONAL ANTIBODY-BASED ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR GLYCYRRHIZIN AND ITS AGLICON, GLYCYRRHETIC ACID

被引：15

作者：

MIZUGAKI, M

ITOH, K

HAYASAKA, M

ISHIWATA, S

NOZAKI, S

NAGATA, N

HANADATE, K

ISHIDA, N

机构：

[1] OHTA NISHINOUCHI HOSP,DIV PHARM,KORIYAMA,FUKUSHIMA 963,JAPAN

[2] MINOPHAGEN PHARMACEUT,RES LAB,ZAMA 228,JAPAN

[3] SENDAI INST MICROBIOL,SENDAI,MIYAGI 980,JAPAN

来源：

JOURNAL OF IMMUNOASSAY | 1994年 / 15卷 / 01期

关键词：

GLYCYRRHIZIN; GLYCYRRHETIC ACID; MONOCLONAL ANTIBODY; INHIBITION ELISA;

D O I：

10.1080/15321819408009569

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Monoclonal antibodies (MoAbs) specific for glycyrrhetic acid (GA) were prepared and characterized. Obtained MoAbs (AGA-1, AGA-3, and AGA-6) reacted with GA dose-dependently, but not with glycyrrhizin (GL), carbenoxolone, and steroids. Next, an enzyme-linked immunosorbent assay (ELISA) system using AGA-1 was established. The standard curve showed good linearity between 0.01 and 1000 ng/ml of GA, and the detection limit was 5 pg/ml. Recovery, and intra- and interassay variations of this assay system was satisfactory. GL was also measurable quantitatively after acid-hydrolysis of the samples. The developed ELISA system would be useful to determine GL and GA in biological samples or drugs as an alternative method to high performance liquid chromatography (HPLC).

引用

页码：21 / 34

页数：14

共 22 条

[1] INTERFERON INDUCTION BY GLYCYRRHIZIN AND GLYCYRRHETINIC ACID IN MICE [J].