ANTIBODY-SYNTHESIS IN ROACH (RUTILUS-RUTILUS) - ANALYSIS OF ANTIBODY-SECRETING CELLS IN LYMPHOID ORGANS WITH ELISPOT-ASSAY

被引：37

作者：

AALTONEN, TM

JOKINEN, EI

VALTONEN, ET

机构：

[1] Department of Biology, University of Jyväskylä

来源：

FISH & SHELLFISH IMMUNOLOGY | 1994年 / 4卷 / 02期

关键词：

RUTILUS-RUTILUS; ANTIBODY-SECRETING CELLS; ANTIBODY SYNTHESIS; ELISPOT-ASSAY; LYMPHOID ORGANS;

D O I：

10.1006/fsim.1994.1011

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The roach (Rutilus rutilus L.) which is a cyprinid fish, was immunised with bovine gamma-globulin (BGG) and the antibody synthesis was studied by counting the number of specific antibody secreting cells (SA.SC) in the spleen and anterior kidney, and by measuring the antibody concentration in the circulation. SASCs and the total number of immunoglobulin secreting cells (TISC) were counted with the ELISPOT (enzyme-linked immunospot) assay, and anti-BGG antibodies and the concentration of immunoglobulin in sera were assayed by ELISA (enzyme-linked immunosorbent assay). The present modification of the ELISPOT-assay takes advantage of biotin-avidin amplification and yields easily detectable and nonfading spots that may be counted even after several weeks of storage. It is also possible to count the number of SASC and TISC in different organs. In this study, immunisation of the roach with protein antigen via the intraperitoneal route resulted in a similar response in the spleen and in the kidney. There was no preferential organ distribution of the number of SASC or TISC and no difference in the timing of the response in the organs. The humoral response due to immunisation in the serum correlated with cellular responses in the organs.

引用

页码：129 / 140

页数：12

共 36 条

[1]

Anderson D.P., Dixon O.W., Immunological memory in rainbow trout to a fish disease bacterin administered by flush exposure, Phylogeny of Immunological Memory, pp. 103-111, (1980)

[2]

Arkhoosh M.R., Kaattari S.L., Development of immunological memory in rainbow trout (Oncorhynchus my kiss). I. An immunochemical and cellular analysis of the B cell response, Developmental and Comparative Immunology, 15, pp. 279-293, (1991)

[3]

Bayer E.A., Wilchek M., The use of the avidin-biotin complex as a tool in molecular biology, Methods of Biochemical Analysis, pp. 2-45, (1980)

[4]

Chart H., Pearson T.W., Trust T.J., Detection of specific fish antibody using an inhibition enzyme-linked immunosorbent assay (Inhibition ELISA), Journal of Immunological Methods, 68, pp. 19-25, (1984)

[5]

Cossarini-Dunier M., Indirect enzyme-linked immunosorbent assay (ELISA) to titrate rainbow trout serum antibodies against two pathogens: Yersinia ruckeri and Egtved virus, Aquaculture, 49, pp. 197-208, (1985)

[6]

Czerkinsky C.C., Nilsson L., Nygren H., Ouchterlony O., Tarkowsky A., A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells, Journal of Immunological Methods, 65, pp. 109-121, (1983)

[7]

Espelid S., Rodseth O.M., Jorgensen T.O., Vaccination experiments and studies of the humoral immune responses in cod, Gadus morhua L. To four strains of monoclonal-defined Vibrio anguillarum, Journal of Fish.Diseases, 14, pp. 185-197, (1991)

[8]

Georgopoulou U., Vernier J.-M., Local immunological response in the posterior intestinal segment of the rainbow trout after oral administration of macromolecules, Developmental and Comparative Immunology, 10, pp. 529-537, (1986)

[9]

Van Ginkel F.W., Van Muiswinkel W.B., Merchant B., Lizzio Dixon E.F.O.W., Anderson D.P., Temperature comparisons for antibody production in vitro by plaque-forming cells from trout, Scarno gairdneri (Richardson), and mice, Journal of Fish Biology, 27, pp. 265-272, (1985)

[10]

Grondel J.L., Nouws J.F.M., Van Muiswinkel W.B., The influence of antibiotics on the immune systems: Immuno-pharmokinetic investigations on the primary anti-SRBC response in carp, Cyprinus carpio L, after Oxytetracycline injection, Journal of Fish Diseases, 10, pp. 35-43, (1987)

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