SPECTRAL CHARACTERIZATION OF NEW CHLOROPHYLL-C PIGMENTS ISOLATED FROM EMILIANIA-HUXLEYI (PRYMNESIOPHYCEAE) BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：25

作者：

GARRIDO, JL ^{[1
]}

ZAPATA, M ^{[1
]}

MUNIZ, S ^{[1
]}

机构：

[1] CONSELLERIA PESCA XUNTA DE GALICIA,CTR INVEST MARINAS,E-36600 VILAGARCIA AROUSA,SPAIN

来源：

JOURNAL OF PHYCOLOGY | 1995年 / 31卷 / 05期

关键词：

CHLOROPHYLL C PIGMENTS; CHLOROPHYLL C(3); EMILIANIA HUXLEYI; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; MAGNESIUM-3,8-DIVINYLPHEOPORPHYRIN A(5) MONOMETHYL ESTER; PELAGOCOCCUS SUBVIRIDIS; PHYTOL-SUBSTITUTED;

D O I：

10.1111/j.0022-3646.1995.00761.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Pigment extracts from the prymnesiophyte Emiliania huxleyi (Lohm.) Hay & Mohler (CCMP 370) were analyzed using a new chromatographic method based on the use of polymeric octadecylsilica columns, which split both the chlorophyll c(3) peak and phytol-substituted chlorophyll c-like peak into two fractions. The pigments corresponding to each of these fractions were isolated. The spectra and chromatographic behavior suggest that chlorophyll c(3) from E. huxleyi is a mixture of two different compounds whereas the splitting of the phytol-substitute c-like pigment could be the result of the equilibrium of two forms showing the same absorption spectrum. The method allowed, as well, the detection of magnesium 3,8-divinylpheoporphyrin a(5) monomethyl ester in the prymnesiophytes E. huxleyi (CCMP 370) and Prymnesium parvum Carter (CCMP 708) and the chrysophyte Pelagococcus subviridis Norris (CCMP 1429).

引用

页码：761 / 768

页数：8

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