DEVELOPING BIOSENSORS WITH PH-ISFET TRANSDUCERS UTILIZING LIPID BILAYER-MEMBRANES WITH TRANSPORT PROTEINS

被引：17

作者：

OTTENBACHER, D ^{[1
]}

KINDERVATER, R ^{[1
]}

GIMMEL, P ^{[1
]}

KLEE, B ^{[1
]}

JAHNIG, F ^{[1
]}

GOPEL, W ^{[1
]}

机构：

[1] MAX PLANCK INST BIOL,DEPT MEMBRANE BIOCHEM,W-7400 TUBINGEN,GERMANY

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1992年 / 6卷 / 1-3期

关键词：

D O I：

10.1016/0925-4005(92)80054-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present a biosensor based on the transport protein lactose permease (LP) which is imbedded in a supported planar lipid bilayer (SPB) membrane, and which allows the potentiometric detection of lactose via pH measurement. Different experimental setups were used to study systematically the electric properties of the different biosensor components. In a first step the preparation of the SPB is investigated by means of high-frequency capacitance-voltage measurements. The sensor consists of an ion-sensitive field-effect transistor (ISFET) coated with the SPB membrane, the latter containing the lactose/H+ cotransporter LP from Escherichia coli (E. coli). The sensor effect consists of a rise in the proton concentration in the small volume between the lipid membrane and the gate area upon addition of lactose to the external medium. This effect is monitored directly by the pH-sensitive ISFET.

引用

页码：192 / 196

页数：5

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