ALTERATIONS OF SINGLE-MOLECULE FLUORESCENCE LIFETIMES IN NEAR-FIELD OPTICAL MICROSCOPY

被引：292

作者：

AMBROSE, WP ^{[1
]}

GOODWIN, PM ^{[1
]}

MARTIN, JC ^{[1
]}

KELLER, RA ^{[1
]}

机构：

[1] LOS ALAMOS NATL LAB,DIV LIFE SCI,LOS ALAMOS,NM 87545

来源：

SCIENCE | 1994年 / 265卷 / 5170期

关键词：

D O I：

10.1126/science.265.5170.364

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Fluorescence lifetimes of single Rhodamine 6G molecules on silica surfaces were measured with pulsed laser excitation, time-correlated single photon counting, and near-field scanning optical microscopy (NSOM). The fluorescence lifetime varies with the position of a molecule relative to a near-field probe. Qualitative features of lifetime decreases are consistent with molecular excited state quenching effects near metal surfaces. The technique of NSOM provides a means of altering the environment of a single fluorescent molecule and its decay kinetics in a repeatable fashion.

引用

页码：364 / 367

页数：4

共 28 条

[1] FLUORESCENCE SPECTROSCOPY AND SPECTRAL DIFFUSION OF SINGLE IMPURITY MOLECULES IN A CRYSTAL [J].