WHOLE-MOUNT ELECTRON MICROSCOPY OF CHROMATIN AND MEMBRANES IN BULL AND HUMAN SPERM HEADS

被引：32

作者：

LUNG, B

机构：

[1] Department of Cell Biology and Pharmacology, University of Maryland, Baltimore

来源：

JOURNAL OF ULTRASTRUCTURE RESEARCH | 1968年 / 22卷 / 5-6期

关键词：

D O I：

10.1016/S0022-5320(68)90036-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The fine structure of bull and human sperm nuclei and membranes was studied by treating unsectioned spermatozoa with alkaline thioglycolate and drying by the Anderson critical point method. The nucleus of bull and human sperm, in an unpacked state, is composed of a network of chromatin fibers. Single fibers range from 140 to 240 Å in diameter and exhibit possible cross fibers or branches. Lengths of measured fibers could be traced for at least 12μ. Three components of an outer membrane system were distinguished in bull sperm after thioglycolate treatment. The human sperm membranes were found to be more labile to thioglycolate treatment. © 1968 Academic Press Inc.

引用

页码：485 / +

页数：1

共 21 条

[1]

ANBERG AKE, 1957, ACTA OBSTET ET GYNECOL SCAND, V36, P1

[2] TECHNIQUES FOR THE PRESERVATION OF 3-DIMENSIONAL STRUCTURE IN PREPARING SPECIMENS FOR THE ELECTRON MICROSCOPE [J].