HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF CREATININE

A high-performance liquid chromatography (HPLC) method for creatinine has been developed. The method is based on ion-pair chromatography. The method is precise and easy to perform. Recoveries from enriched sera were from 98-105% with a mean of 100.5%. At 28-mu-mol 1(-1), 52-mu-mol 1(-1) and 487-mu-mol 1(-1) within-series standard deviations of 2-mu-mol 1(-1), 2-mu-mol 1(-1) and 10-mu-mol 1(-1), respectively were obtained. At 102-mu-mol 1(-1) among-series standard deviation of 6-mu-mol 1(-1) was obtained. Comparisons between HPLC and autoanalyser (Jaffe reaction) methods showed that the autoanalyser plasma and serum results, which are known to be sensitive to 'pseudocreatinines', varied between 93-217% of the HPLC results. Comparisons between HPLC, autoanalyser and enzymatic methods showed that the methods agree well for urine samples. The method presented appears suitable as a secondary reference method.