ESTIMATION OF MINIMAL NUMBERS OF AZOSPIRILLUM-BRASILENSE USING TIME-LIMITED LIQUID ENRICHMENT COMBINED WITH ENZYME-LINKED-IMMUNOSORBENT-ASSAY

被引：11

作者：

BASHAN, Y

MITIKU, G

ZIVVECHT, O

LEVANONY, H

机构：

[1] OHIO STATE UNIV,DEPT AGRON,COLUMBUS,OH 43210

[2] WEIZMANN INST SCI,DEPT PLANT GENET,IL-76100 REHOVOT,ISRAEL

来源：

SOIL BIOLOGY & BIOCHEMISTRY | 1991年 / 23卷 / 02期

关键词：

D O I：

10.1016/0038-0717(91)90125-4

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

A simple time-limited liquid enrichment procedure to aid in the quantitative detection of very few cells of Azospirillum brasilense in plant roots is described. The method is based on limited multiplication of A. brasilense in convential semi-solid medium and counting of the bacteria in the enriched medium by enzyme-linked immunosorbent assay (ELISA) or by Most Probable Number (MPN) techniques. The method can be used as a complementary procedure to ELISA and MPN techniques when low numbers of A. brasilense are present in the roots.

引用

页码：135 / 138

页数：4

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