CHROMOSOME ORGANIZATION OF STREPTOCOCCUS-MUTANS GS-5

被引：16

作者：

HANTMAN, MJ ^{[1
]}

SUN, SZ ^{[1
]}

PIGGOT, PJ ^{[1
]}

DANEOMOORE, L ^{[1
]}

机构：

[1] TEMPLE UNIV,HLTH SCI CTR,SCH MED,DEPT MICROBIOL & IMMUNOL,PHILADELPHIA,PA 19140

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1993年 / 139卷

关键词：

D O I：

10.1099/00221287-139-1-67

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Twenty-eight genetic loci have been physically mapped to specific large restriction fragments of the Streptococcus mutans GS-5 chromosome by hybridization with probes of cloned genes or, for transposon-generated amino acid auxotrophs, with probes for Tn916. In addition, restriction fragments generated by one low-frequency-cleavage enzyme were used as probes to identify overlapping fragments generated by other restriction enzymes. The approach allowed construction of a low resolution physical map of the S. mutans GS-5 genome using restriction enzymes ApaI (5'-GGGCC/C), SmaI (5'-CCC/GGG), and NotI (5'-GC/GGCCGC).

引用

页码：67 / 77

页数：11

共 57 条

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