ORIENTED IMMOBILIZATION OF FAB' FRAGMENTS ON SILICA SURFACES

被引：83

作者：

LU, B

XIE, JM

LU, CL

WU, CG

WEI, Y

机构：

[1] Laboratory of Molecular, Biomolecular Electronics, Southeast University, Nanjing, 210096, P. R.

[2] Department of Chemistry, Southeast University, Nanjing, 210096, P. R

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 01期

关键词：

D O I：

10.1021/ac00097a014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fab' fragments of rabbit anti-human IgG were immobilized in oriented form on derivatized silica surfaces containing pyridyl disulfide groups. The fragments were also covalently coupled in random form to glutaraldehyde-bound silanized silica surfaces, The surfaces were characterized by X-ray photoelectron spectroscopy and ellipsometry. Antigen binding activities of the immobilized Fab' fragments were determined by solid-phase enzyme immunoassay. The results showed that the changes in orientation of the immobilized fragments drastically influence the antigen binding activity of the fragments, and the antigen binding activity of the fragments in oriented form is 2.7 times higher than that in random form. Such immobilized fragments could find extensive applicability in the design of immunosensors for monitoring of soluble antigens.

引用

页码：83 / 87

页数：5

共 23 条

[1] ANTIGEN RECOGNITION PROPERTIES OF ANTIBODY MONOLAYERS [J].