LIQUID-CHROMATOGRAPHIC DETERMINATION OF SODIUM MERCAPTOUNDECAHYDRODODECABORATE IN RAT URINE AND PLASMA AFTER PRECOLUMN DERIVATIZATION

A high-performance liquid chromatographic (HPLC) method was developed for the determination of disodium mercaptoundecahydrododecaborate (BSH) in biological fluids. Monobromobimane was used as a precolumn derivatizing agent. A stable derivative was obtained. The derivative was separated on a C-18 column using reversed-phase ion-pairing chromatography and detected by a spectrophotometric detector at 373 nm. The detection limit was 200 ng/ml (0.1 ppm boron). Calibration curves were prepared for rat urine and plasma samples. The calibration curves were linear in the range of 1 mu g/ml to 100 mu g/ml for urine samples and 0.2 mu g/ml to 50 mu g/ml for plasma samples.