AMPEROMETRIC NADH DETERMINATION VIA BOTH DIRECT AND MEDIATED ELECTRON-TRANSFER BY NADH OXIDASE FROM THERMUS-AQUATICUS YT-1

被引：41

作者：

SOMASUNDRUM, M

HALL, J

BANNISTER, JV

机构：

[1] CRANFIELD UNIV, CTR BIOTECHNOL, CRANFIELD MK43 0AL, BEDS, ENGLAND

[2] UNIV MALTA, DEPT BIOMED SCI, MSIDA, MALTA

来源：

ANALYTICA CHIMICA ACTA | 1994年 / 295卷 / 1-2期

关键词：

AMPEROMETRY; NADH OXIDASE; RUTHENIUM;

D O I：

10.1016/0003-2670(94)80333-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Using NADH oxidase extracted from Thermus aquaticus YT-1, the determination of NADH by the direct oxidation of labile FADH(2) and by mediated electron transfer using the Ru(III/IV) redox couple is described. A scheme for enzyme amplification is outlined using alcohol dehydrogenase (ADH) and NADH oxidase in solution, and enables 1-10 mu M NADH to be determined. Detection is performed at +150 mV using the RuCl62-/RuCl63- couple. Additionally, an electrode is modified by an ADH/NADH oxidase bilayer, enabling ethanol determination (20-200 mu M) at 0 mV via the oxidation of ruthenium red.

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页码：47 / 57

页数：11

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