ANTI-COAGULANT ACTIVITIES OF HIGH AND LOW-MOLECULAR WEIGHT HEPARIN FRACTIONS

被引：129

作者：

BARROWCLIFFE, TW ^{[1
]}

JOHNSON, EA ^{[1
]}

EGGLETON, CA ^{[1
]}

KEMBALLCOOK, G ^{[1
]}

THOMAS, DP ^{[1
]}

机构：

[1] NATL INST BIOL STAND & CONTROL,CHEM SECT,LONDON NW3 6RB,ENGLAND

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1979年 / 41卷 / 04期

关键词：

D O I：

10.1111/j.1365-2141.1979.tb05894.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Summary. The anticoagulant activities of high and low molecular weight heparin fractions were measured by three assay methods, both in vitro, and after intravenous injection in volunteers. The low molecular weight (LMW) fraction had similar anti‐Xa activity in vitro to the high molecular weight (HMW) fraction, but in APTT assays the HMW fraction was about twice as potent. After intravenous injection, the two fractions gave equal heparin levels by anti‐Xa assays, but in APTT assays the LMW fraction had only half the activity of the HMW fraction. Anti‐Xa assays using synthetic substrate S‐222 gave about 20% lower levels than anti‐Xa clotting assays for both heparins. Complete protamine neutralization of the post‐injection heparin activity was found in APTT and synthetic substrate assays, but about 20% of the clotting anti‐Xa effect could not be neutralized. Complete neutralization of the fractions by protamine was shown by all three in vitro. This non‐neutralizable activity probably accounts for the difference between the anti‐Xa clotting and synthetic substrate assays. Studies by crossed immunoclectrophoresis and affinity chromatography indicated that the antithrombin III binding properties of the two fractions were similar. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：573 / 583

页数：11

共 15 条

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