TGF-BETA-1 INDUCES PHOSPHORYLATION OF THE CYCLIC-AMP RESPONSIVE ELEMENT BINDING-PROTEIN IN ML-CC164 CELLS

被引：95

作者：

KRAMER, IM ^{[1
]}

KOORNNEEF, I ^{[1
]}

DELAAT, SW ^{[1
]}

VANDENEIJNDENVANRAAIJ, AJM ^{[1
]}

机构：

[1] NETHERLANDS INST DEV BIOL,HUBRECHT LAB,3584 CT UTRECHT,NETHERLANDS

来源：

EMBO JOURNAL | 1991年 / 10卷 / 05期

关键词：

CRE BINDING; JUN-B; ML-CC164; CELLS; NUCLEAR PROTEIN PHOSPHORYLATION; TGF-BETA-1;

D O I：

10.1002/j.1460-2075.1991.tb08048.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Type beta-transforming growth factors represent a family of polypeptides that modulate growth and differentiation. TGF-beta exerts its effect on target cells through interaction with specific cell surface receptors, but the signal transduction pathways are largely unresolved as yet. In this study we report that TGF-beta-1 induces a rapid phosphorylation of the cyclic AMP responsive element binding protein (CREB) in mink lung CC164 cells. Phosphorylation induced by TGF-beta-1 is not mediated by the cAMP-dependent protein kinase. Parallel to the increase in phosphorylation of CREB, an increase in binding to the collagenase TPA responsive element was observed. CREB participates in the binding to this element, probably as a heterodimer with another as yet unknown protein. The modification imposed on CREB and its involvement in an enhanced TRE-binding could be a mechanism by which TGF-beta-1 induces the TRE-mediated transcriptional activation.

引用

页码：1083 / 1089

页数：7

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