ALPHA-1-ADRENERGIC STIMULATION OF INOSITIDE HYDROLYSIS IN LIVER OF SENESCENT RATS

被引：6

作者：

BORST, SE ^{[1
]}

SCARPACE, PJ ^{[1
]}

机构：

[1] UNIV FLORIDA, DEPT PHARMACOL, GAINESVILLE, FL 32611 USA

来源：

MECHANISMS OF AGEING AND DEVELOPMENT | 1990年 / 56卷 / 03期

关键词：

AGING; ALPHA-ADRENERGIC RECEPTOR; PHOSPHATIDYL INOSITIDES; PHOSPHOLIPASE-C;

D O I：

10.1016/0047-6374(90)90088-W

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Hydrolysis of phosphatidyl inositides (PI) was measured in response to alpha-1-adrenergic agonist in slices of liver prepared from F-344 female rats aged 6-, 15- and 25 months. Tissue was labeled with [H-3]inositol and release of inositol phosphates was measured following a 60-min incubation with a range of concentrations of l-epinephrine. The response to epinephrine was completely blocked by prazosin, indicating the involvement of alpha-1-adrenergic receptors. The maximum response to epinephrine represented release of 13-15% of radioactivity incorporated into the pool of cellular lipids and was unchanged with age. The concentration of epinephrine required for half-maximal stimulation was 1-1.5 mu-M and was also unchanged with age. These data suggest that, although senescence is accompanied by alterations with age in the liver alpha-1-receptor, alpha-1-stimulation of PI hydrolysis is unchanged.

引用

页码：275 / 280

页数：6

共 14 条

[1]

Taylor, Merritt, Receptor coupling to polyphosphatidyl inositol turnover: a parallel with the adenylate cyclase system, Trends Pharmacol. Sci., 7, pp. 238-242, (1986)

[2]

Litosch, Wallis, Fain, 5-Hydroxytryptamine stimulates inositol phosphate production in a cell-free system from blowfly salivary glands

[3]

evidence for a role of GTP in coupling receptor activation to phosphoinositide breakdown, J. Biol. Chem., 260, pp. 5464-5471, (1985)

[4]

Goodhardt, Ferry, Geynet, Hanoune, Hepatic α1 adrenergic receptors show agonist-specific regulation by guanine nucleotides, J. Biol. Chem., 257, pp. 11577-11583, (1982)

[5]

Uhing, Prpic, Jiang, Exton, Hormone-stimulated polyphosphoinositide breakdown in rat liver plasma membranes, J. Biol. Chem., 261, pp. 2140-2146, (1988)

[6]

Morgan, Blackmore, Exton, Age-related changes in the control of hepatic cyclic AMP levels by α1- and β2-adrenergic receptors in male rats, J. Biol. Chem., 258, pp. 5103-5109, (1982)

[7]

Puschel, Jungermann, Activation of inositol phosphate formation by circulating noradrenaline, but not by sympathetic nerve stimulation with a similar increase of glucose release in perfused rat liver, Eur. J. Biochem., 175, pp. 187-191, (1988)

[8]

Katz, McNair, Hymer, Boland, Emergence of beta adrenergic-responsive hepatic glycogenolysis in male rats during post-maturational aging, Biochem. Biophys. Res. Comm., 147, pp. 724-730, (1987)

[9]

Lynch, Taylor, Smith, Exton, Formation of high-affinity agonist state of the α1-adrenergic receptor at cold temperature does not require a G-protein, FEBS Lett., 229, pp. 54-58, (1988)

[10]

Crews, Gonzales, Raulli, McElhaney, Pontzer, Raizada, Interaction of calcium with receptor-stimulated phosphoinositide hydrolysis in brain and liver, Ann. N.Y. Acad. Sci., 522, pp. 88-95, (1988)

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