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EXOGENOUS PRIMER-INDEPENDENT CDNA SYNTHESIS WITH COMMERCIAL REVERSE-TRANSCRIPTASE PREPARATIONS ON PLANT-VIRUS RNA TEMPLATES
被引:12
作者
:
AGRANOVSKY, AA
论文数:
0
引用数:
0
h-index:
0
机构:
MV LOMONOSOV STATE UNIV,AN BELOZERSKY LAB,MOSCOW 119899,USSR
MV LOMONOSOV STATE UNIV,AN BELOZERSKY LAB,MOSCOW 119899,USSR
AGRANOVSKY, AA
[
1
]
机构
:
[1]
MV LOMONOSOV STATE UNIV,AN BELOZERSKY LAB,MOSCOW 119899,USSR
来源
:
ANALYTICAL BIOCHEMISTRY
|
1992年
/ 203卷
/ 01期
关键词
:
Enzymes;
-;
Degradation;
Cloning;
RNA;
D O I
:
10.1016/0003-2697(92)90058-F
中图分类号
:
Q5 [生物化学];
学科分类号
:
071010 ;
081704 ;
摘要
:
Upon reverse transcription and cloning manipulations with virion RNAs of several plant viruses, namely beet yellows virus, brome mosaic virus, and potato virus X, we came across a significant background synthesis of cDNA on the virion RNA template in vitro independent of exogenous primers added. When tested with beet yellow virus RNA template, several commercial preparations of avian myeloblastosis virus (AMV) reverse transcriptase showed the background activity monitored by the [α-32P]dNTP incorporation in vitro, while the enzyme from murine moloney leukemia virus (MMLV) was found strictly exogenous-primer-dependent. To detect possible nucleic acid contaminations in reverse transcriptase, the enzyme preparations from several commercial sources were incubated with [γ-32P]ATP and polynucleotide kinase. The labeled material from AMV reverse transcriptase preparations comigrated with a tRNA marker in polyacrylamide gels and was found to be RNase-sensitive. The MMLV reverse transcriptase preparations were free from such a contamination. These results indicate that the exogenous-primer-independent cDNA synthesis by some AMV reverse transcriptases could be due to a contaminating tRNA (or its low-molecular-weight degradation products) serving as an endogenous primer. © 1992.
引用
收藏
页码:163 / 165
页数:3
相关论文
共 11 条
[1]
NUCLEOTIDE-SEQUENCE OF THE 3'-TERMINAL HALF OF BEET YELLOWS CLOSTEROVIRUS RNA GENOME - UNIQUE ARRANGEMENT OF 8 VIRUS GENES
AGRANOVSKY, AA
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
AGRANOVSKY, AA
BOYKO, VP
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
BOYKO, VP
KARASEV, AV
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
KARASEV, AV
LUNINA, NA
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
LUNINA, NA
KOONIN, EV
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
KOONIN, EV
DOLJA, VV
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
DOLJA, VV
[J].
JOURNAL OF GENERAL VIROLOGY,
1991,
72
: 15
-
23
[2]
PUTATIVE-65 KDA PROTEIN OF BEET YELLOWS CLOSTEROVIRUS IS A HOMOLOG OF HSP70 HEAT-SHOCK PROTEINS
AGRANOVSKY, AA
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
AGRANOVSKY, AA
BOYKO, VP
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
BOYKO, VP
KARASEV, AV
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
KARASEV, AV
KOONIN, EV
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
KOONIN, EV
DOLJA, VV
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
DOLJA, VV
[J].
JOURNAL OF MOLECULAR BIOLOGY,
1991,
217
(04)
: 603
-
610
[3]
3'-TERMINAL LABELING OF RNA WITH T4 RNA LIGASE
ENGLAND, TE
论文数:
0
引用数:
0
h-index:
0
ENGLAND, TE
UHLENBECK, OC
论文数:
0
引用数:
0
h-index:
0
UHLENBECK, OC
[J].
NATURE,
1978,
275
(5680)
: 560
-
561
[4]
A ONE TUBE REACTION FOR THE SYNTHESIS OF BLUNT-ENDED DOUBLE-STRANDED CDNA
GUBLER, U
论文数:
0
引用数:
0
h-index:
0
GUBLER, U
[J].
NUCLEIC ACIDS RESEARCH,
1988,
16
(06)
: 2726
-
2726
[5]
CONSERVED ELEMENTS IN THE 3' UNTRANSLATED REGION OF FLAVIVIRUS RNAS AND POTENTIAL CYCLIZATION SEQUENCES
HAHN, CS
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
HAHN, CS
HAHN, YS
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
HAHN, YS
RICE, CM
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
RICE, CM
LEE, E
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
LEE, E
DALGARNO, L
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
DALGARNO, L
STRAUSS, EG
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
STRAUSS, EG
STRAUSS, JH
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
STRAUSS, JH
[J].
JOURNAL OF MOLECULAR BIOLOGY,
1987,
198
(01)
: 33
-
41
[6]
HUYNH TV, 1987, DNA CLONING, V1, P84
[7]
VIRION RNA OF BEET YELLOWS CLOSTEROVIRUS - CELL-FREE TRANSLATION AND SOME PROPERTIES
KARASEV, AV
论文数:
0
引用数:
0
h-index:
0
机构:
MOSCOW STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR
KARASEV, AV
AGRANOVSKY, AA
论文数:
0
引用数:
0
h-index:
0
机构:
MOSCOW STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR
AGRANOVSKY, AA
ROGOV, VV
论文数:
0
引用数:
0
h-index:
0
机构:
MOSCOW STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR
ROGOV, VV
MIROSHNICHENKO, NA
论文数:
0
引用数:
0
h-index:
0
机构:
MOSCOW STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR
MIROSHNICHENKO, NA
DOLJA, VV
论文数:
0
引用数:
0
h-index:
0
机构:
MOSCOW STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR
DOLJA, VV
ATABEKOV, JG
论文数:
0
引用数:
0
h-index:
0
机构:
MOSCOW STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR
ATABEKOV, JG
[J].
JOURNAL OF GENERAL VIROLOGY,
1989,
70
: 241
-
245
[8]
MANIATIS T, 1982, MOL CLONING
[9]
SPECIFIC BINDING OF TRYPTOPHAN TRANSFER-RNA TO AVIAN-MYELOBLASTOSIS VIRUS RNA-DEPENDENT DNA-POLYMERASE (REVERSE-TRANSCRIPTASE)
PANET, A
论文数:
0
引用数:
0
h-index:
0
机构:
MIT,CTR CANC RES,77 MASSACHUSETTS AVE,CAMBRIDGE,MA 02139
PANET, A
HASELTINE, WA
论文数:
0
引用数:
0
h-index:
0
机构:
MIT,CTR CANC RES,77 MASSACHUSETTS AVE,CAMBRIDGE,MA 02139
HASELTINE, WA
BALTIMORE, D
论文数:
0
引用数:
0
h-index:
0
机构:
MIT,CTR CANC RES,77 MASSACHUSETTS AVE,CAMBRIDGE,MA 02139
BALTIMORE, D
PETERS, G
论文数:
0
引用数:
0
h-index:
0
机构:
MIT,CTR CANC RES,77 MASSACHUSETTS AVE,CAMBRIDGE,MA 02139
PETERS, G
HARADA, F
论文数:
0
引用数:
0
h-index:
0
机构:
MIT,CTR CANC RES,77 MASSACHUSETTS AVE,CAMBRIDGE,MA 02139
HARADA, F
DAHLBERG, JE
论文数:
0
引用数:
0
h-index:
0
机构:
MIT,CTR CANC RES,77 MASSACHUSETTS AVE,CAMBRIDGE,MA 02139
DAHLBERG, JE
[J].
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1975,
72
(07)
: 2535
-
2539
[10]
RAJBHANDARY UL, 1968, J BIOL CHEM, V243, P556
←
1
2
→
共 11 条
[1]
NUCLEOTIDE-SEQUENCE OF THE 3'-TERMINAL HALF OF BEET YELLOWS CLOSTEROVIRUS RNA GENOME - UNIQUE ARRANGEMENT OF 8 VIRUS GENES
AGRANOVSKY, AA
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
AGRANOVSKY, AA
BOYKO, VP
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
BOYKO, VP
KARASEV, AV
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
KARASEV, AV
LUNINA, NA
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
LUNINA, NA
KOONIN, EV
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
KOONIN, EV
DOLJA, VV
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
ACAD SCI USSR,INST MICROBIOL,117811 MOSCOW,USSR
DOLJA, VV
[J].
JOURNAL OF GENERAL VIROLOGY,
1991,
72
: 15
-
23
[2]
PUTATIVE-65 KDA PROTEIN OF BEET YELLOWS CLOSTEROVIRUS IS A HOMOLOG OF HSP70 HEAT-SHOCK PROTEINS
AGRANOVSKY, AA
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
AGRANOVSKY, AA
BOYKO, VP
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
BOYKO, VP
KARASEV, AV
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
KARASEV, AV
KOONIN, EV
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
KOONIN, EV
DOLJA, VV
论文数:
0
引用数:
0
h-index:
0
机构:
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
ACAD SCI USSR,INST MICROBIOL,MOSCOW 117811,USSR
DOLJA, VV
[J].
JOURNAL OF MOLECULAR BIOLOGY,
1991,
217
(04)
: 603
-
610
[3]
3'-TERMINAL LABELING OF RNA WITH T4 RNA LIGASE
ENGLAND, TE
论文数:
0
引用数:
0
h-index:
0
ENGLAND, TE
UHLENBECK, OC
论文数:
0
引用数:
0
h-index:
0
UHLENBECK, OC
[J].
NATURE,
1978,
275
(5680)
: 560
-
561
[4]
A ONE TUBE REACTION FOR THE SYNTHESIS OF BLUNT-ENDED DOUBLE-STRANDED CDNA
GUBLER, U
论文数:
0
引用数:
0
h-index:
0
GUBLER, U
[J].
NUCLEIC ACIDS RESEARCH,
1988,
16
(06)
: 2726
-
2726
[5]
CONSERVED ELEMENTS IN THE 3' UNTRANSLATED REGION OF FLAVIVIRUS RNAS AND POTENTIAL CYCLIZATION SEQUENCES
HAHN, CS
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
HAHN, CS
HAHN, YS
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
HAHN, YS
RICE, CM
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
RICE, CM
LEE, E
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
LEE, E
DALGARNO, L
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
DALGARNO, L
STRAUSS, EG
论文数:
0
引用数:
0
h-index:
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机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
STRAUSS, EG
STRAUSS, JH
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
AUSTRALIAN NATL UNIV,THE FAC,DEPT BIOCHEM,CANBERRA,ACT 2601,AUSTRALIA
STRAUSS, JH
[J].
JOURNAL OF MOLECULAR BIOLOGY,
1987,
198
(01)
: 33
-
41
[6]
HUYNH TV, 1987, DNA CLONING, V1, P84
[7]
VIRION RNA OF BEET YELLOWS CLOSTEROVIRUS - CELL-FREE TRANSLATION AND SOME PROPERTIES
KARASEV, AV
论文数:
0
引用数:
0
h-index:
0
机构:
MOSCOW STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR
KARASEV, AV
AGRANOVSKY, AA
论文数:
0
引用数:
0
h-index:
0
机构:
MOSCOW STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR
AGRANOVSKY, AA
ROGOV, VV
论文数:
0
引用数:
0
h-index:
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机构:
MOSCOW STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR
ROGOV, VV
MIROSHNICHENKO, NA
论文数:
0
引用数:
0
h-index:
0
机构:
MOSCOW STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR
MIROSHNICHENKO, NA
DOLJA, VV
论文数:
0
引用数:
0
h-index:
0
机构:
MOSCOW STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR
DOLJA, VV
ATABEKOV, JG
论文数:
0
引用数:
0
h-index:
0
机构:
MOSCOW STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR
ATABEKOV, JG
[J].
JOURNAL OF GENERAL VIROLOGY,
1989,
70
: 241
-
245
[8]
MANIATIS T, 1982, MOL CLONING
[9]
SPECIFIC BINDING OF TRYPTOPHAN TRANSFER-RNA TO AVIAN-MYELOBLASTOSIS VIRUS RNA-DEPENDENT DNA-POLYMERASE (REVERSE-TRANSCRIPTASE)
PANET, A
论文数:
0
引用数:
0
h-index:
0
机构:
MIT,CTR CANC RES,77 MASSACHUSETTS AVE,CAMBRIDGE,MA 02139
PANET, A
HASELTINE, WA
论文数:
0
引用数:
0
h-index:
0
机构:
MIT,CTR CANC RES,77 MASSACHUSETTS AVE,CAMBRIDGE,MA 02139
HASELTINE, WA
BALTIMORE, D
论文数:
0
引用数:
0
h-index:
0
机构:
MIT,CTR CANC RES,77 MASSACHUSETTS AVE,CAMBRIDGE,MA 02139
BALTIMORE, D
PETERS, G
论文数:
0
引用数:
0
h-index:
0
机构:
MIT,CTR CANC RES,77 MASSACHUSETTS AVE,CAMBRIDGE,MA 02139
PETERS, G
HARADA, F
论文数:
0
引用数:
0
h-index:
0
机构:
MIT,CTR CANC RES,77 MASSACHUSETTS AVE,CAMBRIDGE,MA 02139
HARADA, F
DAHLBERG, JE
论文数:
0
引用数:
0
h-index:
0
机构:
MIT,CTR CANC RES,77 MASSACHUSETTS AVE,CAMBRIDGE,MA 02139
DAHLBERG, JE
[J].
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1975,
72
(07)
: 2535
-
2539
[10]
RAJBHANDARY UL, 1968, J BIOL CHEM, V243, P556
←
1
2
→