THE B1-IMMUNOREACTIVE PROTEINS OF THE PERINATAL SUBMANDIBULAR-GLAND - SIMILARITY TO THE MAJOR PAROTID-GLAND PROTEIN, RPSP

The B1-immunoreactive proteins of type III cells of the perinatal rat submandibular gland are immunologically cross-reactive with proteins of both the sublingual and parotid glands; in particular, protein SMG-A appears similar to a major parotid protein. We isolated SMG-A and the parotid protein (known as MI or leucine-rich protein), prepared polyclonal antibodies to them, and compared their biochemical properties and immunological reactivities. They were identical in their molecular weight on SDS-PAGE (23.5 kDa), tenacious binding to Affi-gel Blue, isoelectric point (pH 4.53). and proteolysis to a 14 kDa peptide: Antibodies to SMG-A showed reactivity with protein SMG-C, a product of the neonatal type I cells, as well as with proteins SMG-BI and SMG-B2, contrasted with the absence of reactivity of anti-M1 IgG with these proteins. Anti-M1 reacted with the ''parotid secretory protein'' (PSP) of the mouse, and M1 appears to be the homologue, in the rat, of mouse PSP.