IN-VITRO STUDY OF TERATOGENIC EFFECTS OF CAFFEINE ON CULTURED RAT EMBRYOS AND EMBRYONIC-CELLS

The teratogenic potential of caffeine was examined in vitro by a whole embryo culture system (WECS) and an embryonic cell culture system (micromas teratogen assay: MTA) in the rat. In the WECS, hyperemia of the tail, and a reducion of the placental size was induced by caffeine at concentrations higher than 50 mug/ml; hypoplasia of the forelimb bud was induced at concentrations higher than 100 mug/ml; hematoma in the yolk sac and dysmorphogenesis of the fore- and hind-limb buds, prosencephalon and tail were induced by 200 mug/m/ caffeine. In the MTA, even with 200 mug/ml caffeine, the toxicological parameters obtained by proliferation and differentiation assays of the midbrain and limb bud cells were almost the same as in the control. In conclusion, caffeine induced various morphological anomalies, but did not affect proliferation or differentiation of cells in these experimental systems.