CHIMERIC ALPHA-BETA OLIGONUCLEOTIDES AS ANTISENSE INHIBITORS OF REVERSE TRANSCRIPTION

被引：23

作者：

BOIZIAU, C

DEBART, F

RAYNER, B

IMBACH, JL

TOULME, JJ

机构：

[1] UNIV BORDEAUX 2, INSERM, U386, MOLEC BIOPHYS LAB, F-33076 BORDEAUX, FRANCE

[2] UNIV MONTPELLIER 2, CHIM BIOORGAN LAB, CNRS, URA 488, F-34095 MONTPELLIER 5, FRANCE

来源：

FEBS LETTERS | 1995年 / 361卷 / 01期

关键词：

RNASE H; ANTISENSE OLIGONUCLEOTIDE; MOLONEY MURINE LEUKEMIA VIRUS;

D O I：

10.1016/0014-5793(95)00138-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alpha-beta chimeric 17-mer oligodeoxyribonucleotides containing either 5, 10 or 15 beta nucleotides were synthesized, The stability of the RNA/chimera hybrids was only slightly affected by the alpha stretch and by the alpha-beta link, as was the affinity of the Moloney Murine Leukemia Virus reverse transcriptase for the duplexes, All chimeras inhibited in vitro cDNA synthesis in a cell-free system to various extent, via the degradation of the RNA target by RNase H.

引用

页码：41 / 45

页数：5

共 23 条

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