3-DIMENSIONAL STRUCTURE OF THE HIGHER-PLANT PHOTOSYSTEM-II REACTION-CENTER AND EVIDENCE FOR ITS DIMERIC ORGANIZATION IN-VIVO

被引：98

作者：

SANTINI, C

TIDU, V

TOGNON, G

MAGALDI, AG

BASSI, R

机构：

[1] UNIV VERONA,IST BIOTECHNOL VEGETALI,I-37100 VERONA,ITALY

[2] UNIV PADUA,DIPARTIMENTO BIOL,PADUA,ITALY

[3] CNR,CTR STUDIO EMOCIANINE & ALTRE MET PROT,PADUA,ITALY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1994年 / 221卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1994.tb18742.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The three-dimensional structure of photosystem II (PSII) has been determined by conventional transmission electron microscopy and computerized three-dimensional reconstruction. Both the complete system and that lacking the oxygen-evolving complex have been analyzed. The PSII complex has a four-lobed structure with twofold symmetry. An estimate of the molecular mass and the results of Deriphat/PAGE analysis suggest that a reaction centre is present in each half of the structure resolved by electron microscopy. Stepwise removal of components of the complex showed that the removal of CP47 (a 47-kDa chlorophyll-protein complex) induces monomerization of PSII, which indicates the importance of this subunit for the dimeric structure.

引用

页码：307 / 315

页数：9

共 38 条

[1] THE ROLE OF THE LIGHT HARVESTING COMPLEX AND PHOTOSYSTEM-II IN THYLAKOID STACKING IN THE CHLORINA-F2 BARLEY MUTANT [J].