DETECTION OF GALACTOSE AND LACTOSE BY A POLY(AMPHIPHILIC PYRROLE)-GALACTOSE OXIDASE ELECTRODE

被引：36

作者：

COSNIER, S

INNOCENT, C

机构：

[1] Laboratoire d'Electrochimie Organique et de Photochimie Rédox, URA Cnrs 1210, Université Joseph Fourier Grenoble 1, 38041, Grenoble Cedex 9

来源：

ANALYTICAL LETTERS | 1994年 / 27卷 / 08期

关键词：

ENZYME SENSOR; POLY(AMPHIPHILIC PYRROLE); OVERLYING POLYMER; AMPEROMETRIC DETECTION; GALACTOSE; LACTOSE;

D O I：

10.1080/00032719408006380

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The entrapment of galactose oxidase (GAO) on an electrode surface by coadsorption with a cationic amphiphilic pyrrole and electropolymerization of this pyrrole monomer is described. This simple and rapid procedure for biosensor construction provides very fast responsive and sensitive GAO-based sensors to galactose and lactose. The electrode response is based on the electrochemical detection of enzymically generated hydrogen peroxide. The stability, optimum pH and selectivity of the bioelectrode as well as the characteristics of the immobilized galactose oxidase have been determined. Poly(amphiphilic pyrrole) films have been electrogenerated on the surface of the bioelectrode and the effect of such additional coatings on the biosensor selectivity have also been examined.

引用

页码：1429 / 1442

页数：14

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