A SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ASSAY FOR TROSPECTOMYCIN, AN AMINOCYCLIOTOL ANTIBIOTIC, IN HUMAN PLASMA AND SERUM

This report describes a sensitive, selective and robust assay for the quantification of trospectomycin an aminocyclitol antibiotic, in human plasma and serum. This is the first published High Performance Liquid Chromatography (HPLC) bioanalytical method for a member of this class of compound. The method involves the selective solid phase extraction of 6’ -n-propyl spectinamycin and the internal standard 6 ’ -n-butyl spectinomycin from 0.5 ml of biofluid, efficient reversed phase high pressure liquid chromatography with post column oxidation, reaction with p-phthaldialdehyde and fluorescence detection. The limit of quantification from 0.5 ml of biofluid is 10 ng/ml. © 1990, Taylor & Francis Group, LLC. All rights reserved.