THE EFFECTS OF ENDOTOXINS ON GAMETES AND PREIMPLANTATION EMBRYOS CULTURED INVITRO

被引：24

作者：

DUMOULIN, JCM

MENHEERE, PPCA

EVERS, JLH

KLEUKERS, APG

PIETERS, MHEC

BRAS, M

GERAEDTS, JPM

机构：

[1] Departments of Obstetrics and Gynaecology, Academic Hospital Maastricht, University of Limburg

[2] Clinical Chemistry, Academic Hospital Maastricht, University of Limburg

[3] Departments of Genetics and Cell Biology, University of Limburg

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 05期

关键词：

ENDOTOXIN; ALBUMIN; HUMAN SPERMATOZOA; FERTILIZATION; MOUSE EMBRYO;

D O I：

10.1093/oxfordjournals.humrep.a137417

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Culture media used for human in-vitro fertilization (IVF) can be contaminated with bacterial endotoxins. All five tested types of commercially available albumin, sometimes used as a protein supplement to IVF media, were shown to contain endotoxins in varying concentrations. Endotoxins are suspected to cause embryo fragmentation and low pregnancy rates in human IVF. However, human sperm viability and the IVF of mouse oocytes and subsequent culture of the zygotes were shown to be unaffected by relatively high endotoxin concentrations. Therefore these techniques cannot be used as quality control assays to detect endotoxins in the IVF culture media.

引用

页码：730 / 734

页数：5

共 25 条

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