ANTIBODY-MEDIATED BACTERIORHODOPSIN ORIENTATION FOR MOLECULAR DEVICE ARCHITECTURES

被引：144

作者：

KOYAMA, K

YAMAGUCHI, N

MIYASAKA, T

机构：

[1] Ashigara Research Laboratories, Fuji Photo Film Co. Ltd., Minamiashigara, Kanagawa

来源：

SCIENCE | 1994年 / 265卷 / 5173期

关键词：

D O I：

10.1126/science.265.5173.762

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A rational method for constructing highly oriented films of purple membrane (PM) has been established by using two kinds of bispecific antibodies with different antigen-binding sites, one binding to a specific side of bacteriorhodopsin and the other to a phospholipid hapten. A hapten monolayer deposited on a metal electrode was treated with a bispecific antibody solution and incubated with a PM suspension to produce a highly oriented PM film, as confirmed by electron microscopy in which an immunogold labeling technique was used. This antibody-mediated PM monolayer was then used in the construction of a light-sensing photoelectric device. A comparison of the two incorporated PM monolayers showed that highly efficient photocurrents were produced by the PM monolayer whose unidirectionally oriented cytoplasmic surface faces the electrode.

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页码：762 / 765

页数：4

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