REGULATION BY GH OF INSULIN-LIKE GROWTH FACTOR-I MESSENGER-RNA EXPRESSION IN RAT EPIPHYSEAL GROWTH PLATE AS STUDIED WITH INSITU HYBRIDIZATION

Expression of insulin-like growth factor I (IGF-I) mRNA and its independence on GH was studied in rat epiphyseal growth plate by in-situ hybridization. Methodological aspects of the technique were first evaluated on fixed sagittal cryosections from 28-day-old rat epiphyseal growth plates to ascertain specific hydridization. In-situ hybridization was compared on sections from 10- and 28-day-old rats and the GH-dependence was studied in 35-day-old hypophysectomized rats. Expression of IGF-I mRNA was apparent in chondrocytes of the proliferative, hypertrophic and early degenerative zones of the growth plate of 28-day-old rats. The epiphyseal growth plate from 10-day-old rats showed a weak hybridization signal compared with 28-day-old rats. In contrast, the mesenchymal cells of the periosteum of 10-day-old rats showed a rather strong hybridization signal. Hypophysectomy resulted in a reduction in hydribization signal and cell number of the growth plate compared with 35-day-old-matched normal rats. GH-Replacement therapy (200 μg human GH s.c. every 4 h for 24 h) resulted in partially restored expression of IGF-I mRNA. The present study has shown that the IGF-I gene is expressed in the rat epiphyseal growth plate chondrocytes and that the expression is dependent on GH. The results support a paracrine/autocrine role of IGF-I for the expression of the stimulatory effect of GH on longitudinal bone growth.