GENOMIC ANALYSIS OF THEILERIA-SERGENTI STOCKS IN JAPAN WITH DNA PROBES

被引：15

作者：

MATSUBA, T

KAWAKAMI, Y

IWAI, H

ONUMA, M

机构：

[1] HOKKAIDO UNIV,FAC VET MED,DEPT EPIZOOTIOL,SAPPORO,HOKKAIDO 060,JAPAN

[2] RAKUNO GAKUEN UNIV,DEPT VET MICROBIOL,EBETSU 069,JAPAN

来源：

VETERINARY PARASITOLOGY | 1992年 / 41卷 / 1-2期

关键词：

D O I：

10.1016/0304-4017(92)90006-U

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Restriction fragment length polymorphisms of Theileria sergenti DNA from 18 different infections of cattle in 14 locations in Japan were analyzed by Southern blotting using T. sergenti genomic DNA fragments as probes. Probe pTs 2 hybridized with four fragments in BamHI digested piroplasm DNA, at 8.0, 7.3, 6.0 and 3.4 kb. Probe pTs 11-D1 hybridized with multiple fragments. With each probe,. polymorphisms were observed among stocks from different locations. However, there was no correlation between the patterns of hybridization bands and the locations where parasites were collected. Analysis of the hybridization patterns of stocks obtained from individual cattle in the same grazing areas showed an almost identical pattern.

引用

收藏

页码：35 / 43

页数：9

相关论文

共 13 条

[1] THEILERIA-PARVA - GENOMIC DNA STUDIES REVEAL INTRA-SPECIFIC SEQUENCE DIVERSITY [J].

ALLSOPP, BA ;

ALLSOPP, MTEP .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1988, 28 (01) :77-83

[2] DNA PROBES DETECT GENOMIC DIVERSITY IN THEILERIA-PARVA STOCKS [J].

CONRAD, PA ;

IAMS, K ;

BROWN, WC ;

SOHANPAL, B ;

OLEMOIYOI, OK .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1987, 25 (03) :213-226

[3] ISOLATION OF THEILERIA PARASITES FROM AFRICAN BUFFALO (SYNCERUS-CAFFER) AND CHARACTERIZATION WITH ANTI-SCHIZONT MONOCLONAL-ANTIBODIES [J].

CONRAD, PA ;

STAGG, DA ;

GROOTENHUIS, JG ;

IRVIN, AD ;

NEWSON, J ;

NJAMUNGGEH, REG ;

ROSSITER, PB ;

YOUNG, AS .

PARASITOLOGY, 1987, 94 :413-423

[4] CHARACTERIZATION OF BUFFALO-DERIVED THEILERIAL PARASITES WITH MONOCLONAL-ANTIBODIES AND DNA PROBES [J].

CONRAD, PA ;

OLEMOIYOI, OK ;

BALDWIN, CL ;

DOLAN, TT ;

OCALLAGHAN, CJ ;

NJAMUNGGEH, REG ;

GROOTENHUIS, JG ;

STAGG, DA ;

LEITCH, BL ;

YOUNG, AS .

PARASITOLOGY, 1989, 98 :179-188

[5] PLASMODIUM-FALCIPARUM - DIFFERENTIATION OF ISOLATES WITH DNA HYBRIDIZATION USING ANTIGEN GENE PROBES [J].

COPPEL, RL ;

SAINT, RB ;

STAHL, HD ;

LANGFORD, CJ ;

BROWN, GV ;

ANDERS, RF ;

KEMP, DJ .

EXPERIMENTAL PARASITOLOGY, 1985, 60 (01) :82-89

[6] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[7]

HIRANO A, 1991, IN PRESS J VET MED S

[8] PLASMODIUM-BERGHEI - GAMETOCYTE PRODUCTION, DNA CONTENT, AND CHROMOSOME-SIZE POLYMORPHISMS DURING ASEXUAL MULTIPLICATION INVIVO [J].

JANSE, CJ ;

BOORSMA, EG ;

RAMESAR, J ;

VANVIANEN, P ;

VANDERMEER, R ;

ZENOBI, P ;

CASAGLIA, O ;

MONS, B ;

VANDERBERG, FM .

EXPERIMENTAL PARASITOLOGY, 1989, 68 (03) :274-282

[9]

KAJIWARA N, 1990, JPN J VET SCI, V52, P1199

[10] ANTIGENIC DIVERSITY OF THEILERIA-ANNULATA MACROSCHIZONTS [J].

SHIELS, B ;

MCDOUGALL, C ;

TAIT, A ;

BROWN, CGD .

VETERINARY PARASITOLOGY, 1986, 21 (01) :1-10