SUBCELLULAR-DISTRIBUTION OF UBIQUITIN PROTEIN CONJUGATES IN THE HIPPOCAMPUS FOLLOWING TRANSIENT ISCHEMIA

被引：38

作者：

HAYASHI, T ^{[1
]}

TAKADA, K ^{[1
]}

MATSUDA, M ^{[1
]}

机构：

[1] SRL INC,DEPT RES LAB,HAJIOCHI,TOKYO 192,JAPAN

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 1992年 / 31卷 / 03期

关键词：

RAT; IMMUNOBLOTTING; NEURONAL CELL DEATH;

D O I：

10.1002/jnr.490310321

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Ubiquitin-protein conjugates in the hippocampus were analyzed by immunoblotting with a monoclonal anti-ubiquitin antibody. In the CA1 region, Triton X-100 insoluble ubiquitin-protein conjugates increased after 24 hr following 20 min of ischemia. When the total hippocampi were fractionated subcellularly, ubiquitin-protein conjugates increased in the particulate, especially in the mitochondrial fraction. The ubiquitin-protein conjugates were solubilized by SDS, or were partially solubilized by urea. The results indicate that insoluble ubiquitin-protein conjugates increase after ischemia.

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页码：561 / 564

页数：4

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