DETERMINATION OF THE RELATIVE CONFIGURATION OF 5,6,7,8-TETRAHYDROMETHANOPTERIN BY 2-DIMENSIONAL NMR-SPECTROSCOPY

被引：21

作者：

SCHLEUCHER, J

SCHWORER, B

ZIRNGIBL, C

KOCH, U

WEBER, W

EGERT, E

THAUER, RK

GRIESINGER, C

机构：

[1] UNIV FRANKFURT,INST ORGAN CHEM,NIEDERURSELER HANG,W-6000 FRANKFURT 50,GERMANY

[2] UNIV MARBURG,MICROBIOL LAB,W-3550 MARBURG,GERMANY

来源：

FEBS LETTERS | 1992年 / 314卷 / 03期

关键词：

5,6,7,8-TETRAHYDROMETHANOPTERIN; RELATIVE CONFIGURATION; HETERONUCLEAR 2-DIMENSIONAL NMR; MOLECULAR MODELING; METHANOGENIC ARCHAEA; METHANOBACTERIUM-THERMOAUTOTROPHICUM;

D O I：

10.1016/0014-5793(92)81522-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The relative configuration of the pterin moiety of 5,6,7,8-tetrahydromethanopterin 1, a coenzyme isolated from methanogenic archaea, has been determined by two-dimensional NMR spectroscopy of N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin 2 to be rel-(6R; 7S; 11R). The complete proton resonance assignment of the pterin moiety of N5,N10-methylene-5,6,7,8-tetrahydromethanopterin 3 is described including the relative stereospecific assignment of the C(14a) methylene protons.

引用

页码：440 / 444

页数：5

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